Mario A. Barón-Rodríguez, PhD

May 31, 2016

Final outcome:

The students will explain the behavior and reactivity of proteins from their structure by making a video. They will understand the laws of interaction of macromolecules in different environments.

Class project: Protein expression of caterpillar Spodoptera fugiperda brain.

​

Videos made by students in 2016-I.

​

"Recycling components of Nickel-Cadmium batteries" by Astrid Carolina Carvaja.

"Extraction, separation and characterization of caterpillar (Spodoptera frugip erda) brain protein" by Natalia Niño and Katherine Vega.

"Brain protein extraction in Spodoptera frugiperda" by Victor Jaramillo and Laura Quiroga.

​

​

April 29, 2016

Purpose: Recognize the enzyme kinetic and the molecular insight of enzyme activity.

Rate reaction in life systems are diverse and complex.  Nature provide rates in soft conditions.  Almost all biological reactions are catalysed by proteins called enzyme.

​

Activity

1. Read the "Measuring Enzyme Behavior: The Michaelis-Menten Equation - Chapter 6. Enzyme Kinetics: Getting There Faster" on Biochemistry for Dummies (Find this book on Ebrary - University database).

2. In pairs, make a mind map related to the modification of Michaelis-Menten equation; depending on the particular conditions.

3. Read the poster "Enantioselectivity Prediction of Candida Antartica B Lipase Towards Racemic Mixture Resolution of (R/S)-propanolol" published on 13th International Bio-Tech Symposium (IBS-2008), China

4.  Write the abstract.

March 28, 2016

Purpose: Be aware of the importance of intermolecular interactions and molecular properties on biotechnological analysis.

In this activity you will find out the importance of intermolecular forces and molecular properties on biotechnological procedures.  This time we are goingo to focus on electrophoresis.

Activity

1. Read Abstract, Introduction and 1D Electrophoresis sessions on "Overview of Protein Elec-

trophoresis", published by ThermoFisher Scientific seller.

2. Watch the video "On separating proteins by SDS-PAGE" on the same page.

3. Ask yourself the next questions:

• Why can you separate molecules by electric field?

• What kind of molecules can be separated?

4. Modelling. Open "electrophoresis.jnlp" by doble click on it.

Make all activities (Gel Electrophoresis) and answer the questions.

5. Answer the following questions:

• What is the structure of SDS? Why is SDS needed to pretreat proteins in Electrophoresis technique?

• Is it important to separate proteins in Environmental Chemistry?

• How do you know that a patient have Sickle Cell trait? Find an article (not more than 10 years old) to support the methodology.

6. Perform activity report, remember to write the abstract (Consider "How to write an Abstract: Some useful tips")

Electrophoresis web pages:

Overview of Protein Electrophoresis by ThermoFisher Scientific

Preparing Protein Samples for Electrophoresis

Electrophoresis

System requirements:

- JAVA and JDK installed on your computer.

- PedagogicaActivities installed on your computer.  Download here. Instructions are in "Read me.txt".

- Download electrophoresis.jnlp activity.

February 23, 2023

Purpose: Reinforce the learned concepts of Intermolecular Forces by using interactive simulations.

1. Open "Molecular Workbench" (MW) software. Click on "Browse all activities..">"Intermolecular attractions" (It is in Chemistry topic).

2. Do all "Intermolecular attraction" activities.  Hand in the report.

3. In pairs discuss about:

• How could molecular model (in MW) be created to simulate an acetic acid sorption on charcoal?

• How could molecular model (in MW) be created to simulate a diffusion of Methylene Blue, Potassium Dichromate or Copper Sulfate?

• Which other environmental problems could be simulated at molecular level?

Share your answer in the classroom.

4. Answer the Southeastern Louisiana University’s test about Intermolecular Forces. Show your results to the professor and discuss the answer in class.